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Tandem mass tags (TMT or TMTs) are chemical labels used for mass spectrometry (MS)-based quantification and identification of biological macromolecules such as proteins, peptides and nucleic acids. TMT belongs to a family of reagents referred to as isobaric mass tags. They provide an alternative to gel- or antibody-based quantification but may also be used in combination with these and other methods. ==Versions== There are currently four varieties of TMT available: * TMTzero – a non-isotopically substituted core structure * TMTduplex – an isobaric pair of mass tags with a single isotopic substitution〔 * TMTsixplex – an isobaric set of six mass tags with five isotopic substitutions * TMT 10-plex - a set of 10 isotopic mass tags which use the TMTsixplex reporter region, but use different elemental isotope to create a mass difference of approximately 0.01 Da The tags contain four regions, namely a mass reporter region (M), a cleavable linker region (F), a mass normalization region (N) and a protein reactive group (R). The chemical structures of all the tags are identical but each contains isotopes substituted at various positions, such that the mass reporter and mass normalization regions have different molecular masses in each tag. The combined M-F-N-R regions of the tags have the same total molecular weights and structure so that during chromatographic or electrophoretic separation and in single MS mode, molecules labelled with different tags are indistinguishable. Upon fragmentation in MS/MS mode, sequence information is obtained from fragmentation of the peptide back bone and quantification data are simultaneously obtained from fragmentation of the tags, giving rise to mass reporter ions. Proteome Sciences Plc have the patents for TMT technology. These patents have broad coverage of all tandem mass tag technology. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Tandem mass tag」の詳細全文を読む スポンサード リンク
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